Chemoautotrophy, symbiosis and sedimented diatoms support high biomass of benthic molluscs in the Namibian shelf.

The molluscs Lucinoma capensis, Lembulus bicuspidatus and Nassarius vinctus are highly abundant in Namibian oxygen minimum zone sediments. To understand which nutritional strategies allow them to reach such impressive abundances in this extreme habitat we investigated their trophic diversity, including a chemosymbiosis in L. capensis, focussing on nitrogen biochemical pathways of the symbionts. We combined results of bulk nitrogen and carbon (δ13C and δ15N) and of compound-specific isotope analyses of amino acid nitrogen (AAs-δ15NPhe and δ15NGlu), with 16S rRNA gene sequencing of L. capensis tissues and also with exploratory results of ammonium, nitrate and nitrite turnover. The trophic position (TP) of the bivalve L. capensis is placed between autotrophy and mixotrophy, consistent with its proposed symbiosis with sulfur-oxidizing Candidatus Thiodiazotropha sp. symbionts. The symbionts are here revealed to perform nitrate reduction and ammonium uptake, with clear indications of ammonium host-symbionts recycling, but surprisingly unable to fix nitrogen. The TP of the bivalve L. bicuspidatus is placed in between mixotrophy and herbivory. The TP of the gastropod N. vinctus reflected omnivory. Multiple lines of evidences in combination with current ecosystem knowledge point to sedimented diatoms as important components of L. bicuspidatus and N. vinctus' diet, likely supplemented at times with chemoautotrophic bacteria. This study highlights the importance of benthic-pelagic coupling that fosters the dietary base for macrozoobenthos in the OMZ. It further unveils that, in contrast to all shallow water lucinid symbionts, deeper water lucinid symbionts rely on ammonium assimilation rather than dinitrogen fixation to obtain nitrogen for growth.

Community bioirrigation potential (BIPc), an index to quantify the potential for solute exchange at the sediment-water interface.

BIOIRRIGATION: the animal-induced exchange of solutes between pore water and overlying water - is a key process in sediments with profound implications for biogeochemical processes such as nutrient cycling and organic matter regeneration at the sediment water interface. There is an urgent need to understand how a changing environment will affect the irrigation activity of macrofauna and vice versa. A shift in species composition (e.g. from deep burrowing species to smaller, more opportunistic and shallow burrowing species) will have large effects on bioirrigation and thus on ecosystem function (such as benthic pelagic coupling). Considering the difficulties to determine area-covering rates of bioirrigation (e.g. in terms tracer-based fluxes) and the complexity of interactions of multiple species in the community that prohibit a direct measure of bioirrigation attributable to each species, a mechanistically-based approach is needed to predict relative intensities of bioirrigation activity based on the fundamental functional traits. We propose a conceptual framework to develop an index of bioirrigation that takes into account the biological mechanisms of bioirrigation and provides a simplified, yet functionally based approach to quantify the bioirrigation potential of benthic communities. We developed the community bioirrigation potential (BIPc) that provides a biomass- and abundance-weighted scoring system considering functional traits related to pore water and solute exchange. It may be used as a supplement to established methods to assess the function of marine soft sediments related bioirrigation. In analogy to the particle-related community bioturbation potential of Solan et al. (2004), context dependent organismal traits that affect ventilation and bioirrigation (feeding type, morphology of burrows, and burrowing depth) are combined with the data on abundance and biomass of the respective species. These are subsequently summed up to a community bioirrigation potential (BIPc). This review considers ecological traits relevant for bioirrigation and their classification into a bioirrigation index. Furthermore the necessary simplifications in the index (e.g. limiting its applicability to interfacial nutrient fluxes) are discussed. We also provide a working example from the southwestern Baltic Sea to illustrate the practical application of the index and a compilation of key species related to this area containing their classification into the considered bioirrigation traits.

Glacial-driven vicariance in the amphipod Gammarus duebeni.

We have examined the genetic diversity using mitochondrial COI and ND2 sequence data from 306 specimens of the amphi-Atlantic-distributed amphipod Gammarus duebeni. Marine populations from the Atlantic Ocean, the Baltic and North Sea, as well as freshwater populations from Ireland, Cornwall and Brittany were analysed. G. duebeni is a complex of five allopatric lineages. Freshwater populations result from multiple invasions of marine ancestors, represented by distinct lineages. We interpret the recent distribution of lineages as the outcome of a series of spatio-temporal vicariant events caused by Pleistocene glaciations and sea level changes. The freshwater lineages are therefore regarded as 'glacial relicts'. Furthermore, inter-specific competition with, for example, Gammarus pulex (which is absent in Ireland and western Brittany) may be another important determinant in the distribution of freshwater G. duebeni. In Ireland and Brittany, three freshwater refugia are suggested. The significantly limited gene flow detected among marine populations is more likely due to inter-specific competition than to salinity. The G. duebeni-complex represents a model system for the study of allopatric speciation accompanied by major habitat shifts. The pattern of spatio-temporal origins of the freshwater entities we describe here provides an excellent system for investigating evolutionary adaptations to the freshwater environment. Our data did not confirm the presently used subspecies classification but are only preliminary in the absence of nuclear genetic analyses.

Long-term exposure of several marine benthic animals to static magnetic fields.

Electrical currents in underwater sea cables could induce magnetic fields. The sea cables lie on or within the sea bottom and this is the living area for many invertebrate and vertebrate species. North Sea prawn Crangon crangon (Crustacea, Decapoda), round crab Rhithropanopeus harrisii (Crustacea, Brachyura), glacial relict isopod Saduria entomon (Crustacea, Isopoda), blue mussel Mytilus edulis (Bivalvia), and young flounder Plathichthys flesus (Pisces) were exposed to a static magnetic field (MF) of 3.7 mT for several weeks. The results showed no differences in survival between experimental and control animals. Mussels M. edulis were kept under static magnetic field conditions for 3 months during their reproductive period in spring. The determination of gonad index and condition index revealed no significant differences to the control group.